A sensitive and reliable detection of thrombin via enzyme-precipitate-coating-linked aptamer assay.
نویسندگان
چکیده
Stable enzyme precipitate coatings (EPCs) with high enzyme loading were employed to develop a sensitive and reliable detection protocol, termed EPC-linked aptamer assay (EPC-LAA). EPC-LAA achieved the limit of detection to be 0.5 ng per mL of thrombin (13.5 pM), and maintained a reasonably stable detection signal even after storing all the reagents at 40 °C for 66 days.
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ورودعنوان ژورنال:
- Chemical communications
دوره 48 48 شماره
صفحات -
تاریخ انتشار 2012